Fig 1: Validation of enriched genes shared by CSCs and EPCs or specific for CSCs by immunofluorescence and telomere FISH of CRCs. a) Proliferation markers PCNA or MKI67 and shared markers KRT18, SLC12A2, CXADR, and CLDN4. b) Proliferation marker MKI67 costained with CSC enriched genes, TNFRSF19 and BAMBI. White arrows indicate marker positive but MKI67 negative cells. c) CSC specific markers TNFRSF19 or BAMBI (green) and telomere FISH (red). Areas within dashed lines in orange indicate marker positive cells; areas within dashed lines in white indicate marker negative cells. d) CSC and EPC shared markers SLC12A2 or KRT18 and telomere FISH (red). Areas within dashed lines in orange indicate marker positive cells; areas within dashed lines in white indicate marker negative cells. Scale bar = 20 µm.